Fig. (3) Reduction in Initiation of Transcription during Noncytolytic CD8+ T cell mediated Suppression of HIV Replication. Both JR-HVS and HS-HVS significantly suppressed viral transcription initiation more than the HIV naŃ—ve CD8+ T cells (nEW CD8+), p < 0.001 for both comparisons, Student t-Test. The fold difference between the suppressed and nonsuppressed viral cultures were determined by quantifying cDNA copy numbers from the short RNA transcripts using real time PCR.