Table 1.:
Primer Sequence [5’to 3’] 5’ Position [bp]
HIVshortF CCTGGGAGCTCTCTGGCTAA 29
HIVshortR AACAGACGGGCACACACTACTTT 119
3kb-F CAATGACATACAGAAATTAGTGGGAAA 2868
3kb-R CTTTGGTTCCCCTAAGAAGTTTACAT 2958
7kb-F TGGCAGGAAGTAGGAAAAGCA 7040
7kb-R AGCAGCCCAGTAATATTTGATGAAC 7120
Luc-F AGGTGGCTCCCGCTGAA *9.6 kb
Luc-R ACACCTGCGTCGAAGATGTTG *9.7 kb
RPS9-F GACGGGGAAGCGGAGCCAACA **308
RPS9-R1 GCTTCAGCTCTTGGTCGAGACGAG **418
RPS9-R2 GCGAGCGTGGTGGATGGACTTG **5579

* Primer locations are based on the luciferase gene sequence in Promega’s pGL-3 basic vector, forward primer at 1343 bp and reverse primer 1406 bp. The luciferase reporter gene is located in the nef gene of the HIV pNL4-3.LUC.R-E- genome at 8.3 kb.
** Primer locations are based on Ensembl Gene ID: ENSG00000170889.