Fig. (1) (A) Casp8p41 induced apoptosis is independent of caspase 8 activity. Jurkat and I 9.2 cells were transfected with plasmids containing GFP or GFP-casp8p41 for 8 hours. Cells were harvested and mitochondria transmembrane potential measured on the GFP-positive cells by TMRE staining. (B) Pool results of three replicates of Fig. (1A). (C) Jurkat cells were transfected with plasmids containing GFP or GFP-casp8p41, and immediately after transfection, cells were incubated with the indicated caspase inhibitors for 8 hours. Cells were harvested and cell viability determined using a cell titer glow luminescent assay.