Fig. (1) Schematic of genetic construction of plasmid pAd5/H3-HVR2-His (GL). The gene of interest AscI-H3-HVR2-His-BstXI was digested with restriction enzymes AscI and BstXI, and ligated into the shuttle plasmid H3pH5S to form H3/pH5S-HVR2-His. H3/pH5S-HVR2-His was then digested with EcoRI and PmeI, resulting in the production of a fragment containing homologous arms for recombination and the gene of interest. The fragment was then recombined with SwaI-linearized pAd5/ΔH5 (GL) by interaction of homologous arms. The resulting recombinant plasmid was named pAd5/H3-HVR2-His (GL).