Fig. (4) Induction of antigen-specific humoral immunity by chimeric vector via the “Antigen Capsid-Incorporation” strategy. (A) Schema of “prime-boost” regimen in BALB/c mice. The solid lines with arrow denote the time of prime, boost, and reboost. The dotted lines with arrow denote the time of bleeding post injection. (B) KDI peptide (LQIGHHHHHHKDITTTE) was coated in the sera-based ELISA to test the binding of the peptide to sera from the four immunization groups. The groups analyzed were unimmunized mice, Ad3, Ad5H3, Ad5H3-HVR2-His and Ad5H3-HVR2-His + Adjuvant. The values in the ELISA assays were expressed as the Mean ± SD, representing seven independent replicates. Statistical analyses were performed using One-way ANOVA.