Fig. (4) The alignment analysis identified two cross-over events. The cross-over events in the 123bp conserved region (4A) and 20bp conserved region (4B) were identified. The recombinants are identical to GFPm in region-1 but identical to EGFP in region-3, which were highlighted in red dotted line box. The conserved and cross-over regions are in region-2.