Table 1: Primers used in this study.

PCR Name Sequence*(5'-3')
First round Univ-A-Rec-F1 TCTCGGTTCTGCATTCGACCCCGTCATTGAACCAACTTT
Univ-B-Rec-R1 ATTCGCTGGCACGCACTTTGTAGTTGCCGTCGTCCTTGA
Univ-A-Rec-F2 TCTCGGTTCTGCATTCGATCGTGACCACCCTGACCTAC
Univ-B-Rec-R2 ATTCGCTGGCACGCACTTCGTTGTGGCTGTTGTAGTTGTA
Univ-A-Rec-F3 TCTCGGTTCTGCATTCGATACAACTACAACAGCCACAACG
Univ-B-Rec-R3 ATTCGCTGGCACGCACTTTGTTCCGCTGAAACTCTGGT
Second round A-KMID1-Univ-A CCATCTCATCCCTGCGTGTCTCCGACTCAGACACGACGACTTCTCGGTTCTGCATTCGA
B-K-Univ-B CCTATCCCCTGTGTGCCTTGGCAGTCTCAGATTCGCTGGCACGCACTT
* The universal tails are highlighted in bold, 454 adaptor sequences are bold and underlined, the key sequences are italic, the multiplex identifier (MID) is underlined, and targeted gene sequences are shown in regular.