| PCR | Name | Sequence*(5'-3') |
|---|---|---|
| First round | Univ-A-Rec-F1 | TCTCGGTTCTGCATTCGACCCCGTCATTGAACCAACTTT |
| Univ-B-Rec-R1 | ATTCGCTGGCACGCACTTTGTAGTTGCCGTCGTCCTTGA | |
| Univ-A-Rec-F2 | TCTCGGTTCTGCATTCGATCGTGACCACCCTGACCTAC | |
| Univ-B-Rec-R2 | ATTCGCTGGCACGCACTTCGTTGTGGCTGTTGTAGTTGTA | |
| Univ-A-Rec-F3 | TCTCGGTTCTGCATTCGATACAACTACAACAGCCACAACG | |
| Univ-B-Rec-R3 | ATTCGCTGGCACGCACTTTGTTCCGCTGAAACTCTGGT | |
| Second round | A-KMID1-Univ-A | CCATCTCATCCCTGCGTGTCTCCGACTCAGACACGACGACTTCTCGGTTCTGCATTCGA |
| B-K-Univ-B | CCTATCCCCTGTGTGCCTTGGCAGTCTCAGATTCGCTGGCACGCACTT | |
| * The universal tails are highlighted in bold, 454 adaptor sequences are bold and underlined, the key sequences are italic, the multiplex identifier (MID) is underlined, and targeted gene sequences are shown in regular. | ||