Table 4: Multiple nucleotide sequence alignments. The alignments were performed using the Clustal W2 sequence alignment method through MEGA X program. The table describe 99% nucleotides similarity between isolates 2,5,6,7,8 and USA isolate with some point mutations that either to be silent which can be exemplified by isolate 7,6 and 2 in position 262,264 and 261 respectively or transversion mutation in isolates 5,8 which reflect a mutation in position 276 that result in Amino acid Substitution.
|
261 ' ' | ' ' ' ' ' 270 ' ' ' | ' ' ' ' ' 280 ' ' |
|
Usanc001538
|
T |
G |
T |
T |
C |
C |
A |
C |
C |
A |
C |
T |
G |
A |
C |
A |
A |
T |
G |
A |
T |
G |
|
isolate8
|
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
C |
. |
. |
. |
. |
. |
. |
|
isolate7
|
. |
C |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
|
isolate6
|
. |
. |
. |
C |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
|
isolate5
|
. |
A |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
C |
. |
. |
. |
. |
. |
. |
|
isolate2
|
A |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |
. |