Table 5: Multiple sequence amino acids alignments. The alignments was done by using Clustal W2 sequence alignment method through MEGA X software. isolates 1,3 and 4 were showed an identical amino acids similarity with isolates from IRAN, JAPAN, GERMANY, USA and ZAMBIA with Gen Bank accession number of GU130546.1, LC185075.1, NC_025892, CAP-m9-USA-AY628233 and NC_025896 respectively.

Isolates	58   '   60   '   '   '   '  |  '   '   '   '   70   '   '   '   '   |   '   '   80
germenync025892	Y	L	D	G	S	V	K	N	K	R	T	Q	I	F	P	V	E	D	P	K	D
cap m9usa628233	.	.	F	.	.	.	.	.	.	I	.	.	.	.	.	Y	.	.	.	.	.
jpan lc185075.1	.	.	.	S	.	.	N	.	.	.	.	.	.	.	.	.	.	.	.	.	.
iran 130546.1	L	.	.	.	.	.	.	.	.	.	.	R	.	.	.	.	.	.	.	.	.
zambianc 025896	.	.	.	.	.	.	.	.	.	.	.	.	V	.	.	.	.	.	.	.	.
isolate1	*	*	*	*	*	*	*	*	*	*	*	*	*	*	.	.	.	.	.	.	.
isolate3	*	*	L	*	*	*	*	*	*	*	*	*	*	*	.	.	.	.	.	.	.
isolate4	*	C	*	*	*	*	*	*	*	*	*	*	*	*	.	.	.	.	.	.	.