Table 6: Multiple sequence amino acids alignments. The alignments was done by using Clustal W2 sequence alignment method through MEGA X software. a transvertion mutation was observed in position 253 for isolate 5 and 8 when compared to USA 001538 isolate sequences. While isolate 2, 6 and 7 have similar amino acids sequence similarity with USA 001538 isolate.

Isolates	170   '   '   '   '   '   245   '   '   '   '   250   '   '   '   '   255   '
USAnc001538		Q	H	S	T	P	I	A	L	T	R	D	P	Y	N	N	L	D	S
Isolate2		*	*	*	*	*	*	.	.	.	.	.	.	*	*	*	*	*	*
Isolate7		*	*	*	*	*	*	.	.	.	.	.	.	*	*	*	*	*	*
Isolate 6		.	.	.	.	.	*	*	*	*	*	*	*	*	*	*	*	*	*
Isolate5		*	*	*	*	*	*	*	*	*	*	*	*	*	.	T	.	.	.
Isolate8		*	*	*	*	*	*	*	*	*	*	*	*	*	.	T	.	.	.