Fig. (4) LANAp is transactivated by the Spm2 and other E1A-encoding plasmids. MV3 cells were transfected with the WT LANAp construct using 1 µg of the pGL3-LANAp plasmid and the indicated amounts of the respective E1A constructs. Cells were harvested 48 h later and luciferase expression was measured. C = VP22 control plasmid. Relative luciferase activity is depicted. Data represent three independent experiments. The transfection efficiency was 50 ± 10%. *p< 0.005; §p < 0.001; #p<0.05.