Table 1: Titer of TPV-GFP from Infected Cancer Cell Lines at 7 Days Post Infection

Cell Lines Total Virus Yield in 5 ml
Control (Owl Monkey Kidney) cells OMK 1.0125 x 106
Human Breast Cancer Cells T47D 7.25 x 104
MCF-7 1.7375 x 105
MDA-MB-435 5.25 x 105
Human Glioblastoma Cells U-118 2.875 x 105
U-373 2.25 x 107
U-87 4.375 x 103
Human Osteosarcoma Cells HOS 5.5 x 104
Human Melanoma Cells SK-MEL5 4.25 x 103
Human Ovarian Cancer Cells SK-OV-3 1.9625 x 105
Human Renal Cancer Cells ACHN 7.75 x 106
Caki-1 4.0 x 106
Human Colorectal Cancer Cells COLO205 4.5 x 104
HCT-116 1.05 x 107
Human Prostate Cancer Cells PC3 6.5 x 104

Cells were grown in 25 cm2 flasks to confluency and each flask was infected with approximately 1 X 105 pfu. Infected flasks were incubated at 37° C for 7 days, and total virus yield was quantitated as described in Materials and Methods.