Fig. (2) DGCR8- or Dicer-null ESCs. DGCR8-null ESCs lack differentiation ability, and, in turn DGCR8 is essential for the silencing of self-renewal. Dicer-null ESCs also lack differentiation ability. But both null cells remain alive and pluripotent markers still continue to be expressed. In Fig. (1B), there is self-renewal set of human miRNAs (miR-371 cluster in C19MC), suggesting that the resident miRNAs are present independently and the resident miRNAs might play important role in the ESCs’ self-renewal program. These data are based on the criteria of the RNA wave.