Fig. (4) HuR overexpression in Huh7-D12 cells. (A) Western blot analysis of HuR protein in Huh7 and Huh7-D12 cells. Total protein extracts were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and incubated with anti-HuR and anticlathrin antibodies. Images are representative of three independent experiments. (B) The blots were digitalized and band intensity was calculated using the ImageJ program. Relative expression levels were obtained after normalization with the housekeeping clathrin protein.