Fig. (2) Tricine-SDS-PAGE of NSP4ΔN72 and ΔN112 polypeptides from different strains. 2A, Lanes 1-16 represent NSP4 of SA11, RRV, Hg18, NCDV, FRV99, EHP, EC, Wa, S2, ST3, 1040, I321, 116E, N136, 2KD/851 strains and N136-E158K mutant protein. Note that analysis of ΔN72 from only 15 strains and one mutant protein is shown in the figure. Faint bands corresponding to dimmer (D) and tetramer (T) are shown by arrows. 2B, NSP4ΔN112 peptides from 6 different strains. M, Pre-stained MW markers (Biorad).