Table 2.: Semi-Automated Antibody Detection Techniques

Vendor System Marker Method Characteristics
BIORAD EVOLIS Antibodies ELISA/3rd generation/Sandwich Response time: 2 h
Can be fully automated on EVOLIS
Connection to Laboratory Information System (LIS)
Antibodies + Antigen ELISA/4th generation/Sandwich Response time: 2 h 30 min
Analytical sensitivity for HIV Ag: 13.6 pg/ml
Can be fully automated on EVOLIS
Connection to Laboratory Information System (LIS)
p24 antigen ELISA/Sandwich Response time: 3 h
Can be fully automated on EVOLIS
IZASA BEST 2000 Antibodies 3rd generation HIV ELISA Response time: 2 h
Can be fully automated on Best 2000 or any analyser
Connection to Laboratory Information System (LIS)
Antibodies + Antigen 4 generation ELISA Response time: 2 h 30 min
Can be fully automated on Best 2000 or any analyser
Connection to SIL
DIA SORIN ETI-MAX Antibodies 3rd generation ELISA/Sandwich Response time: 2 h
Can be fully automated on Eti-Max.
Connection to Laboratory Information System (LIS)
Antibodies +Antigen ELISA/4th generation/Sandwich Response time: 2 h 30 min
Analytical sensitivity for HIV Ag: 16 pg/ml
Can be fully automated on Eti-Max.
Connection to Laboratory Information System (LIS)
SIEMENS BEP III, BEP 2000 Antibodies ELISA/3rd generation/Sandwich Response time: 3 h
Can be fully automated on Bep III/2000.
Connection to Laboratory Information System (LIS)
Disposable tips
Antibodies +Antigen ELISA/4th generation/Sandwich Response time: 3 h
Can be fully automated on BepIII/2000, Connection to SIL
Disposable tips
Bidirectional connection
Analytical sensitivity between 100 and 250 pg for p24 Ag