| Abbott RealTime HIV-1 (m2000rt) | COBAS® AmpliPrep/COBAS® TaqMan HIV-1, v2.0 (Roche) | NucliSens® EasyQ HIV-1 v1.2 (bioMérieux) | VERSANT® HIV-1 RNA 1.0 (kPCR) (Siemens) | Artus HI Virus-1 QS-RGQ (Qiagen) | |
|---|---|---|---|---|---|
| HIV target region | Highly conserved region within the pol gene (integrase) | Dual target: highly conserved region of the gag gene (p41) and LTR | Highly conserved region within the gag gene (p24) | Highly conserved region within the pol gene (integrase) | 5LTR region |
| Internal control | Yes. Non-competitive | Yes | Yes. Non-competitive | Yes | Yes |
| Amplification | Real-time PCR | Real-time PCR (TaqMan) | Real-time PCR (NASBA) | Real-time PCR (TaqMan) | Real-time PCR |
| Detection | Fluorescence | Fluorescence | Fluorescence - Molecular beacons | Fluorescence | Fluorescence |
| Quantification | Copies/ml, log10 copies/ml, IU/ml or log10 IU/ml; conversion factor to IU/ml is 1 IU=0.56 copies and 1 copy=1.74 IU | Copies/ml, log10 copies/ml; conversion factor to IU/ml is 1 IU=0.6 copies and 1 copy=1.7 IU | Copies/ml; conversion to IU 1:1 | Copies/ml; IU/ml | UI/ml; conversion factor 1 IU=0.46 copies and 1 copy=2.17 IU |
| Linear dynamic range | 40 copies/ml from 600 l 11 million copies/ml |
20 copies/ml from 850 l 10 million copies/ml |
24 copies/ml 1 million copies/ml |
37 copies/ml 11 million copies/ml |
112.5 copies/ml 45 million copies/ml |
| Specificity (%) (95%) | 100 (99.28-100) | 100 (99.3-100) | 100 | 99.7 (99.3-100) | 100 |
| Subtypes/HIV-2 | Group M -subtypes A-D, F-H, various CRFs, including CRF01_AE and CRF02_AG; group N, O and P. Does not detect HIV-2. |
Group M -subtypes A-D, F-H, various CRFs, including CRF01_AE; group O Does not detect HIV-2. |
Group M -subtypes A-D, F-H, J Does not detect HIV-2. |
Group M -subtypes A-D, F-H, CRF01_AE and CRF02_AG; group O Does not detect HIV-2. |
Group M -subtypes A-D, F-H Does not detect HIV-2. |
| Control of contamination with AmpErase | No | AmpErase (UNG) | No | AmpErase (UNG) | No |