|
Abbott RealTime HIV-1 (m2000rt) |
COBAS® AmpliPrep/COBAS® TaqMan HIV-1, v2.0 (Roche) |
NucliSens® EasyQ HIV-1 v1.2 (bioMérieux) |
VERSANT® HIV-1 RNA 1.0 (kPCR) (Siemens) |
Artus HIVirus-1 QS-RGQ (Qiagen) |
Instrumentation |
m2000sp + integrated computer and m2000rt + integrated computer |
COBAS Ampliprep + TaqMan 48 + computer |
EasyMAG (extraction) + computer and EasyQ + computer |
Sample extraction module (SP module) + amplification and detection module (AD module) |
QIAsymphony SP/AS + Rotor-Gene Q + integrated computer |
Ease of use |
Fully automated, very easy to load. Automated extraction equipment separate from amplification/detection equipment |
Simple to use, very easy to load. Equipment integrated onto one platform |
Easy to use. Automated extraction equipment separate from amplification/detection equipment, manual pipetting of master mix |
Fully automated. Extraction equipment separate from amplification/detection equipment |
Automated extraction equipment separate from amplification/detection equipment |
Reading of primary tube by barcode |
Yes |
No |
Yes |
Yes |
Yes |
Pipetting steps |
Add the internal control to the extraction buffer prior to loading the reagents |
Pipetting of the samples into the Roche equipment tubes |
Additional pipetting steps during the extraction and amplification |
Not required |
Preparation of the internal control-RNA carrier mixture |
Connection to LIS2
|
Yes, bidirectional |
Yes, bidirectional |
Yes, bidirectional |
Yes, bidirectional |
Yes, bidirectional |