| GS-FLX (454)/ GSJunior | SOLEXA | ABI SOLID | |
|---|---|---|---|
| Company | Roche | Illumina | Applied Byosystems |
| Sequencing method | Polymerase (pyrosequencing) | Polymerase (reversible terminators) | Ligase (octamers with two-base encoding) |
| PCR technique | Emulsion PCR | Bridge PCR | Emulsion PCR |
| Read Length (pb) | 250-400 | 35-75 | 25-75 |
| Run time (h) | 10 | 48 | 168 |
| nt/run (Gb) | 0.4 | 18 | 30 |
| Advantages | Amplicon size | Cheap | Cheap |
| Disadvantages | Homopolymers and manual | No de novo and manual | No de novo and manual |