Table 2.: Fully-Automated Assays for Detection and Identification of Respiratory Viruses

Assay Manufacturer Method Virus Detected Hands-On/ Turnaround Times (min)
MariPOC® respi test ArcDia Antigen detection Flu A, Flu B, RSV, hMPV, PIV1-3, ADV < 1 /20-120
Xpert® Flu Cepheid Nucleic acid extraction
Real-time multiplex RT-PCR
Flu A, Flu B, 2009 H1N1 1 / 60
SimplexaTM Flu A/B & RSV Direct kit Focus Diagnostics Nucleic acid extraction
Real-time multiplex RT-PCR
Flu A, Flu B, RSV 1 / 60
Verigene® Respiratory virus plus test Nanosphere Nucleic acid extraction
RT-PCR
Nanoparticle probe-based DNA-array hybridization
Flu A, Flu B, RSV A, RSV B, 2009 H1N1, Flu A H1, Flu A H3, oseltamivir-resistant 2009 H1N1 (H275Y mutation) 5 / 150
Verigene® Respiratory virus XP test FluA, FluB, RSV A, RSV B, 2009 H1N1, Flu A H1, Flu A H3, oseltamivir-resistant 2009 H1N1 (H275Y mutation), PIV 1-4, ADV, hMPV, HRV, HEV 5 / 90
Filmarray® Respiratory Panel Idaho technology Inc. Nucleic acid extraction
RT
Multiplex nested-PCR
Detection by end-point melting curves
ADV, hBoV, HCoV (HKU1, NL63, 229E, OC43), Flu A H1, 2009 H1N1, Flu A H3, Flu B, hMPV, PIV1-4, RSV, HRV/HEV 2 / 60