Sample | CSCs Markers | % Tumor Cells with CSCs Marker | Min. CSCs to Engraft | Recapitulation of Original Tumor Phenotype | Serial Transplant Performed | Additional Assays | Ref. |
---|---|---|---|---|---|---|---|
Normal cervix and CIN I-II-III | p63 CK17 | * | - | - | - | - | Martens, 2004 [32] |
Normal cervix and CSCC | Nanog Musashi-1 Nucleostemin |
Normal cervix < 65% and CSCC > 90%, with all markers** | - | - | - | Correlation with clinicopathological features | Ye, 2008 [39] |
Normal cervix and CSCC | PSCA PIWIL1 TBX2 |
Normal cervix < 9% and CSCC > 50% | - | - | - | Correlation with clinicopathological features and HPV16 | Liu, 2010 [40] |
Normal cervix, CIN-III, CSCC, cell lines and spheres | Sox2 | SiHa and spheres from primary cancer tissues: positive. Normal cervix: 25%, CIN III: 83%, CSCC: 77% | 106 SiHa-Sox2 cells | Yes | No | Proliferation and clonogenicity of exogenous Sox2 in Hela and SiHa cell lines | Ji, 2010 [42] |
Invasive carcinomas and adenocarcinomas | ALDH | Invasive squamous carcinoma 25% and adenocarcinomas 20% | - | - | - | CD133 expression | Yao, 2011 [45] |
SiHa and Ca Ski cell lines | Spheres ALDH |
CaSki: 32% SiHa: 0% Ca Ski: 30% SiHa:24% |
- | - | - | A431 sphere characterization | Bortolomai, 2010 [46] |
HeLa cell line | Spheres ALDH1 CD44 |
HeLa-Twist elevate the level of SFE, ALDH1 and CD44 in HeLa | - | - | - | Activation of -catenin and Akt pathways by Twist and suppression of CD44 expression by inhibition of signaling pathways. | Li, 2011 [51] |
CSCC | Spheres | - | 105 | Yes | No | Chemoresistance assay Expression of embryonic and stemness genes | Feng, 2009 [52] |
HeLa, SiHa, Ca Ski and C33A cell lines | Spheres | HeLa: 18% | 104 | - | No | Chemoresistance assay and gene silencing of E6 in HeLa sphere-forming cells | Gu, 2011 [53] |
HeLa, SiHa, Ca Ski and C-4I cell lines | Spheres-CD49f | 4-12% | 103 | Yes | Yes | Gene expression analysis and radioresistance assay | López, 2012 [44] |