Fig. (1) Establishment of a stable Luc-Con1-NS5A-YFP subgenomic replicon cell line.A. Schematic diagram of the dual luciferase/YFP reporter subgenomic Con1 replicon. B. The YFP-positive cell population was enriched using the BD FACSAria sorter. C. Sustained replication of the sub-genomic replicon measured via luciferase in Huh 7.5.1 Luc-Con1-NS5A-YFP cells over the period of one month is nearly identical to Huh 7.5.1 Luc-Con1-NS5A cells. D. Titration of Alisporivir in Huh 7.5.1 Luc-Con1-NS5A YFP cells demonstrates a direct correlation between luciferase and YFP levels, t tests conducted between both data sets indicate no statistical significant difference, in each data set P>0.05. Error bars represent SD. E. Luc-Con1-NS5A-YFP Huh-7.5.1 cells were visualized via confocal analysis 5 days post drug treatment with Alisporivir (2 µM) and SCY-635 (2 µM).