Fig. (2) Screening of inhibitors of HCV replication using the flow cytometry live cell-based assay.A. Luc-Con1-NS5A-YFP Huh-7.5.1 cells (500,000) were exposed to anti-HCV agents (2 µM) and analyzed for YFP content 3 days post-drug treatment. B. Same as A except that cells were analyzed for YFP content after days 1, 2, 3 and 4. C. Same as B except that the drug was used at 3 concentrations: 2, 0.2 and 0.2 µM. Results (A-C) are representative of 3 independent experiments. D. Same as 1B. E. Same as 2A except that 2 and 3 days post-drug exposure cells were lysed and analyzed for viral and host protein content by Western blotting as described previously [31].