Table 1.: Inhibitory and Cytotoxic Effects of Antivirals on Luc-Con1-NS5A-YFP Subgenomic Replicon Replication

Treatment EC50 (nM) CC50 (I M)
Telaprevir 8.1 >20
BMS-790052 1.4 >20
CsA 311 4.9
Alisporivir 7.9 >20
SCY-635 8.2 >20
SfA 288 10.1
BC544 6.1 >20

EC50 is the concentration of drugs required to reduce the replicon content in Huh7.5.1 cells by 50% as measured by luciferase activity in cell lysates. CC50 is the concentration of drug required to inhibit cell proliferation by 50% as determined by an MTS assay. EC50 and CC50 were determined as previously described [28]. Results are representative of 3 independent experiments.