Fig. (2) Coomassie blue stained gel of expressed proteins isolated from Cos7 or E. Coli cells. COS7 cells were transfected with pcDNA3.1myc/his-142R, pcDNA3.1myc/his, or pcDNA3.1myc/his-142R-GFP (kinase dead) and subsequently affinity purified. p53 was isolated from E. coli cells transformed with p3113 GST-p53 using a glutathione-agarose column. Proteins were separated on a 12% SDS-PAGE and stained with Coomassie brilliant blue.