| Primer |
Sequence |
PCR Parameters |
Amplicon Size (bp) |
| HPV16E6/E7F |
5’-GTGGACCGGTCGATGTATGTCT-3’ |
1 |
209 |
| HPV16E6/E7R |
5’-TCCGGTTCTGCTTGTCCAGC-3’ |
| HPV16L1F |
5’-AGCACGGATGAATATGTTGCACG-3’ |
148 |
| HPV16L1R |
5’-CCCTGTATTGTAATCCTGATACTTTAG-3’ |
| HPV18E6/E7F |
5’-AGTGCCATTCGTGCTGCAAC-3’ |
2 |
286 |
| HPV18E6/E7R |
5’-GGCTTCACACTTACAACACA-3’ |
| HPV18L1/L2F |
5’-GATTATTTACAAATGTCTGCA-3’ |
118 |
| HPV18L1/L2R |
5’-GCACAGTGTCACCCATAGTA-3’ |
| HPV45L2F |
5’-TGTTGGACATCACACCTACCGTGGA-3’ |
3 |
205 |
| HPV45L2R |
5’-TCCGTACCTGACCCAGAAGATGCAA-3’ |
| HPV53E6/E7F |
5’-ATATGTGGACCGGGTCGTGC-3’ |
143 |
| HPV53E6/E7F |
5’-GGCATTGCAGGTCAATCTCAGT-3’ |
| GAPDH-F |
5’-CATTGACCTCAACTACATGGT-3’ |
4 |
130 |
| GAPDH-R |
5’-TCGCTCCTGGAAGATGGTGAT-3’ |
The PCR Parameters used, were as follows; (1) 95°C x 15 min 8 cycles: 94°C x 1 min, 61°C- 1 °C/cycle x 1.5 min, 72°C x 1 min; 37 cycles: 94°C x 1 min, 55°C x 1 min, 72°C x 1
min; 72°C x 7 min, (2) 95°C x 15 min; 8 cycles: 94°C x 1 min, 60°C -1 °C/cycle x 1.5 min, 72°C x 1 min; 37 cycles: 94°C x 1 min, 53°C x 1 min, 72°C x 1 min; 72°C x 7 min, (3)
95°C x 15 min; 8 cycles: 94°C x 1 min, 64°C- 1 °C/cycle x 1.5 min, 72°C x 1 min; 37 cycles: 94°C x 1 min, 57°C x 1 min, 72°C x 1 min; 72°C x 7 min (4) 94°C x 5 min; 33 cycles:
94°C x 25 s, 53°C x 25 s, 72°C x 25 s; 72°C x 7 min.