Fig. (4) Cytosolic E1 reduces CHOP activation. HeLa cells were
transfected with plasmids encoding the envelope proteins as
indicated with or without treatment with the solvent control DMSO
or 2µg/ml tunicamycin (Tm) for 16hr. Protein lysates resolved on
SDS-PAGE were immunoblotted with antibodies specific for
CHOP and β-tubulin. The intensities of the bands were quantified
with ImageQuant 5.0. The levels of CHOP were normalised against
β-tubulin and expressed as fold change relative to the gfp control
which was set as 1.