Fig. (4) Actin depolymerizer cytochalasin D negatively affects HSV-1 glycoprotein mediated cell fusion with zebrafish (ZF) encoded- 3-OST-3 receptor. In this experiment target CHO-K1 cells expressing either human 3-OST-3 or ZF encoded 3-OST-3 along with luciferase gene were pre-incubated with Cyto D (0.5 µg/ml) or with 1 x PBS for 45 min at room temperature before co-culturing with effector cells expressing four essential HSV-1 glycoproteins (gB, gD, gH-gL) or control plasmid (pCDNA3.1) along with T7 Polymerase in 1:1 ratio for 24 hrs. The graph shows the quantitative cell fusion in presence (line bar) and absence of Cyto D (plain bar). The black bar shown is a negative control. Data represent the means standard deviations of results in triplicate wells from a representative experiment. ** P< 0.05, one way ANOVA.