The Open Bioactive Compounds Journal


ISSN: 1874-8473 ― Volume 8, 2020

Marine Streptomyces Sp. VITMK1 Derived Pyrrolo [1, 2-A] Pyrazine-1, 4-Dione, Hexahydro-3-(2-Methylpropyl) and Its Free Radical Scavenging Activity

Manickavelu Manimaran, Krishnan Kannabiran*
VIT University - Department of Biomedical Sciences, Vellore, Tamil Nadu, India



Free radical generation has been proved to be responsible for various cellular diseases. It is necessary to combat free radicals using antioxidants derived from natural sources.


The objective of this study is to evaluate the antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 isolated from mangrove sediment soil collected from Pichavaram, Tamil Nadu, India.


The antioxidant potential of pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl) (diketopiperazine) extracted from Streptomyces sp. VITMK1 was studied using reducing power assay. The scavenging of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and nitric oxide (NO) radical by the compound was also studied. The cytotoxic activity of the compound on RAW 264.7 macrophage cell line was studied using MTT cell viability assay.


The compound exhibited strong DPPH radical scavenging activity (72.48±0.32% at 500 µg/mL) and NO radical scavenging activity (73.03±1.02% at 500 µg/mL). MTT cell viability assay revealed that the compound exhibited concentration-dependent cell viability and was observed to be 92% at 125 µg/mL concentration.


The antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 can be probed further to establish its radical scavenging activity.

Keywords: DPPH radical scavenging assay, Nitric oxide scavenging activity, Pyrrolo [1, 2-A] pyrazine-1, 4-dione, Hexahydro-3-(2-methylpropyl)–, Reducing power assay.

Article Information

Identifiers and Pagination:

Year: 2017
Volume: 5
First Page: 23
Last Page: 30
Publisher Id: TOBCJ-5-23
DOI: 10.2174/1874847301705010023

Article History:

Received Date: 19/06/2017
Revision Received Date: 15/08/2017
Acceptance Date: 18/08/2017
Electronic publication date: 26/09/2017
Collection year: 2017

© 2017 Manimaran and Kannabiran.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the VIT University, Department of Biomedical Sciences, Vellore, Tamil Nadu, India; Tel: +91-416-2202477; E-mail:

Track Your Manuscript:


"It is an honor for us to publish our article in Open bioactive compound journal. The journal follows single blind peer review and plagiarism checking policy which is appreciable. I think soon your journal will attain high impact factor journals."

Zubair K. Labu
Department of Pharmacy, World University of Bangladesh, Bangladesh

Browse Contents

Webmaster Contact:
Copyright © 2020 Bentham Open