On bases of previous observations on the band 3 anion exchange inhibition by the electrophilic reagent 1-fluoro-2,4-dinitrobenzene in resealed ghosts and a previously proposed band 3-based mechanism of control of the erythrocyte shape, it is suggested that stomatocytoses by 1-chloro-2,4-dinitrobenzene and other electrophilic reagents are due to an inhibition of the band 3 anion exchange by a covalent modification of Lys 539, one of the two lysine intramolecularly cross-linked by impermeant 4,4’-diisothiocyanodihydrostilbene-2,2’- disulfonic acid, a band 3 anion exchange potent inhibitor. Attempts to explain these stomatocytoses led to explain the echinocytosis and competitive inhibition of band 3 anion exchange by stilbenedisulfonic acid derivatives and the reversal of the echinocytosis by 2,4-dinitrophenol by one stomatocytogenic of the electrophilic reagents. The inference on stomatocytoses by electrophilic reagents is of interest since stomatocytogenic and echinocytogenic by amphiphiles are perceived to interact non-covalently with the membrane and that they can be clinically relevant.
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