The applications of immunoglobulin preparation for intravenous injection (IVIg) for various intractable diseases
are increasing. The two major clinical indications for IVIg are the replacement therapy and the anti-inflammation
therapy for a variety of acute and chronic autoimmune diseases. One of the proposed mechanisms of IVIg activity is the
modulation of cytokine expression and function; therefore, we analyzed the effect of IVIg on pathogen-associated molecular
pattern (PAMP)-induced cytokine production by peripheral blood mononuclear cells (PBMCs). The production of
tumor necrosis factor-α (TNF-α) as a result of stimulation with lipopolysaccharide (LPS), polyinosinic-polycytidylic acid
sodium salt (Poly I:C), or Pam3CysSerLys4 (Pam3) was significantly inhibited by sulfonated-IVIg (S-IVIg), or by F(ab')2.
Assessed by one-color microarray analysis, the expressions of 229 genes were inhibited to 1/200 or less by F(ab')2. On the
other hand, the expressions of 159 genes were increased by more than 100-fold by F(ab')2. According to these results, it
was suggested that IVIg inhibits inflammatory PAMPs-induced cytokine production by PBMCs, due to the modulation of
varieties of gene expression.