The antinociceptive activity of mycelial extract from fermented mushroom of Ganoderma lucidum, Coprinus
comatus and Grifola frondosa were studied in this paper. The writhing test, formalin test and hot-plate test were used to
evaluate the antinociceptive activity of the acetone extracts of the three Species of Fungi of Basidiomycetes in the study
respectively. The extract of Ganoderma lucidum inhibited in a dose-dependent manner the acetic acid-induced abdominal
constrictions in mice (p<0.05). Also both phases of the response were significantly inhibited in the formalin test (p<0.05).
In the hot-plate test, results showed that extract of Ganoderma lucidum significantly inhibited the reaction time to thermal
stimuli at 30, 60, and 90 min (p<0.05). The preliminary toxicologic study demonstrated the safety of it. However the extract
from fermented mushroom of Coprinus comatus and Grifola frondosa did not show significantly expectative antinociceptive
effect. Our results show the acetone extract of Ganoderma lucidum is a potent analgesic drug.