Centrin1 (CETN1) is a member of the EF-hand superfamily of calcium-binding proteins that localizes to centrosomes
and regulates cytokinesis. Four centrin genes have been reported in mice, of which one, CETN1, is specifically
expressed in the mouse testis. Computer-assisted analysis showed that CETN1 was predominantly expressed in the testis.
In this study, human CETN1, CETN2, and CETN3 mRNAs from various organs were subjected to reverse transcription
polymerase chain reaction (RT-PCR) analysis. CETN1 was specifically detected only in the testis. Next, we identified polymorphisms
of human CETN1 (centrin1) as a candidate gene in human male infertility. To investigate the possible association
between CETN1 and male infertility, DNA from 282 infertile male patients and 96 male volunteers proven to be
fertile was screened for mutations in CETN1. Ten polymorphisms were found in the open reading frame and the 3' untranslated
region of CETN1. These data provide a basis for further large-scale genetic analysis of the association between
genetic background and male infertility.