The Open Bioactive Compounds Journal




ISSN: 1874-8473 ― Volume 7, 2019

Colon and Breast Anti-cancer Effects of Peptide Hydrolysates Derived from Rice Bran



Arvind Kannan1 , Navam Hettiarachchy1, *, Satya Narayan2
1 Department of Food Science, University of Arkansas, Fayetteville, Arkansas
2 Department of Anatomy and Cell Biology and UF Shands Cancer Center, University of Florida, Gainesville, Florida

Abstract

Rice bran is an economical, under-utilized co-product of rough rice milling. The objective of this study was to produce rice-bran peptides and investigate for anti-cancer activity. Protein hydrolysates were prepared by treating heat stabilized defatted rice-bran with food grade Alcalase enzyme, followed by treatment with simulated gastric and intestinal juices to obtain resistant peptides. Resistant peptides were fractionated into >50, 10-50, 5-10, and <5 kDa sizes, freeze dried, and evaluated for inhibitory and cytotoxicity activities on human colon (HCT-116) and breast (HTB-26) cancer cell lines. The results showed that <5 kDa fraction of rice-bran is a potent anti-cancer agent. The cytotoxicity of the fraction to both cancer cell types was more pronounced after the treatment with 500 µg/mL. The IC50 of the peptide fraction was approximately 750 µg/mL. These results indicate that the <5 kDa peptide fraction separated from rice bran protein hydrolysate has a potent anti-tumor activity for colon cancer cells. The peptide fractions that demonstrate anti-cancer activities have the potential for use as functional food ingredients for health benefits.



Article Information


Identifiers and Pagination:

Year: 2009
Volume: 2
First Page: 17
Last Page: 20
Publisher Id: TOBCJ-2-17
DOI: 10.2174/1874847300902010017

Article History:

Received Date: 24/12/2008
Revision Received Date: 20/1/2009
Acceptance Date: 14/2/2009
Electronic publication date: 16/4/2009
Collection year: 2009

Article Metrics:

CrossRef Citations:
0

Total Statistics:

Full-Text HTML Views: 1762
Abstract HTML Views: 1264
PDF Downloads: 331
ePub Downloads: 134
Total Views/Downloads: 3491

Unique Statistics:

Full-Text HTML Views: 1030
Abstract HTML Views: 709
PDF Downloads: 245
ePub Downloads: 101
Total Views/Downloads: 2085
Geographical View

© Kannan et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.


* Address correspondence to this author at the Department of Food Science, University of Arkansas; E-mail: nhettiar@uark.edu



Endorsements



"It is an honor for us to publish our article in Open bioactive compound journal. The journal follows single blind peer review and plagiarism checking policy which is appreciable. I think soon your journal will attain high impact factor journals."


Zubair K. Labu
Department of Pharmacy, World University of Bangladesh, Bangladesh


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2019 Bentham Open