1 Moltech-Anjou, University of Angers/UMR 6200, 2 bd Lavoisier, 49045 Angers Cedex 01, France
2 MitoLab Unit, MITOVASC Institute, UNIV Angers, INSERM U1083, CNRS 6015, UBL Université Bretagne Loire, Angers F-49933 , France
3 CHU, Cellular and Tissular Department, 49033 Angers Cedex 01, France
4 SCIAM, IBS (PBH-IRIS), 4, rue Larrey, 49033 Angers Cedex, France
5 Wroclaw University of Technology, Faculty of Chemistry, Department of Polymer Engineering and Technology, 50-370, Wroclaw, Poland
6 Micro & Nanomédecines Translationelles-MINT, UNIV Angers, INSERM U1066, CNRS UMR 6021,UBL Université Bretagne Loire, Angers, F-49933, France
Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.
Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.
Results & Discussion:
We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).
Keywords: Polymer, PC12 cells, SEM, AFM, Time Laps, Video Microscopy.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Address correspondence to this author at the Department of Neurobiology and neuropathology, CHU and MINT, IBS (PBH-IRIS)° 4 rue Larrey, 49033 Angers Cedex, France, Tel: 33 (0) 241354735; E-mail: firstname.lastname@example.org