The Open Biomedical Engineering Journal

ISSN: 1874-1207 ― Volume 13, 2019

Characterization of Cells Interactions with Patterned Azopolymer-Based Materials using SEM, AFM and Video Microscopy

Régis Barillé1, Philippe Codron2, 3, Guillaume Mabilleau4, Florence Manero4, Romain Mallet4, Sonia Zielinska5, Ewelina Ortyl5, Joël Eyer6, Franck Letournel3, 6, *
1 Moltech-Anjou, University of Angers/UMR 6200, 2 bd Lavoisier, 49045 Angers Cedex 01, France
2 MitoLab Unit, MITOVASC Institute, UNIV Angers, INSERM U1083, CNRS 6015, UBL Université Bretagne Loire, Angers F-49933 , France
3 CHU, Cellular and Tissular Department, 49033 Angers Cedex 01, France
4 SCIAM, IBS (PBH-IRIS), 4, rue Larrey, 49033 Angers Cedex, France
5 Wroclaw University of Technology, Faculty of Chemistry, Department of Polymer Engineering and Technology, 50-370, Wroclaw, Poland
6 Micro & Nanomédecines Translationelles-MINT, UNIV Angers, INSERM U1066, CNRS UMR 6021,UBL Université Bretagne Loire, Angers, F-49933, France



Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.


Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.

Results & Discussion:

We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).

Keywords: Polymer, PC12 cells, SEM, AFM, Time Laps, Video Microscopy.

Article Information

Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 92
Last Page: 100
Publisher Id: TOBEJ-12-92
DOI: 10.2174/1874120701812010092

Article History:

Received Date: 02/08/2018
Revision Received Date: 8/11/2018
Acceptance Date: 14/11/2018
Electronic publication date: 30/11/2018
Collection year: 2018

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© 2018 Letournel et al

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Neurobiology and neuropathology, CHU and MINT, IBS (PBH-IRIS)° 4 rue Larrey, 49033 Angers Cedex, France, Tel: 33 (0) 241354735; E-mail:


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