The Open Biochemistry Journal




ISSN: 1874-091X ― Volume 13, 2019
RESEARCH ARTICLE

Micro-RNAs -106a and -362-3p in Peripheral Blood of Inflammatory Bowel Disease Patients



Ameneh Omidbakhsh1, Mohsen Saeedi2, Masoud Khoshnia3, Abdoljalal Marjani4, *, Safoura Hakimi1
1 Student Research Committee, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran
2 Stem Cell Research Center, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran
3 Golestan Research Center of Gastroenterology and Hepatology, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran
4 Metabolic Disorders Research Center, Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran

Abstract

Objective:

MicroRNAs (miRNAs) can regulate various genes after binding to target mRNAs. Studies on Inflammatory Bowel Disease (IBD) in relation with miRNA are much less shown. The aim of the present study was to assess the expression patterns of microRNA 106a and microRNA 362-3p in peripheral blood samples of Inflammatory Bowel Disease (IBD) patients including Crohn’s Disease(CD) and Ulcerative Colitis (UC).

Methods:

This study consisted of 32 CD, 32 UC patients and 32 controls. The expression level of the micro-RNAs -106a and -362-3p was determined using reverse transcription and real-time RT-PCR.

Results:

Our findings showed that MiR-106a and miR-362-3p are expressed at significantly higher levels in the peripheral blood from patients with CD and UC compared to controls. MiR-106a and miR-362-3p expression are also different in the peripheral blood ofpatients regarding the activity score of the disease. There were significant differences of miR362-3p in active UC relative to inactive UC.

Conclusion:

Altogether our findings suggest that miR-106a and miR-363-3p can play an important role in the pathogenesis of IBD. The differences in expression of miR106a and miR362-3p in peripheral blood of the UC and CD patients in an active phase in comparison to inactive disease suggest that these miRNAs may be useful as potential biomarkers for diagnosis and monitoring the disease activity.

Keywords: MicroRNA-106a, MicroRNA-362-3p, Inflammatory Bowel Disease, Biomarkers, Crohn's Disease, Ulcerative Colitis.


Article Information


Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 78
Last Page: 86
Publisher Id: TOBIOCJ-12-78
DOI: 10.2174/1874091X01812010078

Article History:

Received Date: 15/03/2018
Revision Received Date: 01/06/2018
Acceptance Date: 04/06/2018
Electronic publication date: 29/06/2018
Collection year: 2018

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© 2018 Omidbakhsh et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Metabolic Disorders Research Center, Department of Biochemistry and Biophysics, Faculty of Medicine, GolestanUniversity of Medical Sciences, Gorgan, Golestan province, Iran; Tel/Fax: +98(171)4421651 & 4440225. Zip code: 4934174515; E-mail: abdoljalal@yahoo.com


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