The Open Biochemistry Journal




ISSN: 1874-091X ― Volume 13, 2019

Identification of Metastasis Associated Antigen 1 (MTA1) by Serological Screening of Prostate Cancer cDNA Libraries



Geng Li*, 1, Deepak P Assudani#1, Aija Line2, Fuming Cao1, Amanda Miles1, Robert C Rees1, Stephanie E.B McArdle*, 1
1 School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK
2 Biomedical Research and Study Centre, University of Latvia, Latvia, LV-1067

Abstract

Over the past 10 years the serological analysis of recombinant cDNA expression libraries (SEREX) has proved to be an effective method for the identification of tumour antigens. In the present study, two prostate cancer libraries were constructed and screened using autologous sera. Fifty five genes were isolated, including 46 known genes and 9 previously uncharacterised genes. Among the known genes, a metastasis-associated gene, MTA1, previously identified by differential cDNA hybridisation, was preferentially expressed in a panel of malignant tissues compared with normal tissues, as analysed by reverse transcriptase-polymerase chain reaction (RT-PCR). MTA1 transcripts were observed to be over-expressed in normal human testes as well as various cancer tissues when compared to the panel of normal tissues. MTA1 antigen reacted with 2 of 13 allogeneic prostate cancer patient sera tested, but no sera reactivity was observed to any of the normal adult sera tested. Furthermore, a similar distribution and expression level of MTA-1 was observed in murine tissues and cancer cell lines. Based on these findings and previous reports on the literature on this gene, MTA-1 can be considered not only as a “biomarker” of aggressive disease but also as a potential therapeutic target.

Keywords: Tumour antigen, SEREX, EST and MTA1.


Article Information


Identifiers and Pagination:

Year: 2008
Volume: 2
First Page: 100
Last Page: 107
Publisher Id: TOBIOCJ-2-100
DOI: 10.2174/1874091X00802010100

Article History:

Received Date: 27/5/2008
Revision Received Date: 12/6/2008
Acceptance Date: 20/6/2008
Electronic publication date: 2/7/2008
Collection year: 2008

Article Metrics:

CrossRef Citations:
0

Total Statistics:

Full-Text HTML Views: 763
Abstract HTML Views: 583
PDF Downloads: 236
Total Views/Downloads: 1582

Unique Statistics:

Full-Text HTML Views: 488
Abstract HTML Views: 369
PDF Downloads: 151
Total Views/Downloads: 1008
Geographical View

© Li et al.; Licensee Bentham Open.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.


* Address correspondence to this author at the School of Biomedical and Natural Sciences, Nottingham Trent University, Clifton Lane, Nottingham, NG118NS, UK; Tel: +44(0)115 848 6684; Fax: +44(0)115 848 3384; E-mail: stephanie.mcardle@ntu.ac.uk# These authors contributed equally to the manuscript.



Endorsements



"We have always had a fruitful cooperation and long-term experience of publishing with Bentham Open."

"A number of our original papers have appeared in its different journals, as the Bentham Open Publishers provides sufficient research in the field of science and technologies (belonging to the pharmaceutical, biotechnology, biomedical industries etc). The staff of the Bentham Open always treat our papers very seriously and gently during the rutile paper reviewing process, so far we always felt their kindness and highly cooperative support. Today, Bentham Open is providing a great support to science and research, giving opportunities of high standard publishing as well as promoting and enhancing selected papers around the world through communication and exchange of global scientific education."


Kholmirzo T. Kholmurodov
Leading Scientist
FLNP (Frank Laboratory of Neutron Physics) JINR (Joint Institute of Nuclear Research) Moscow region, Russia


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2019 Bentham Open