1 Department of Biology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway
2 Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, N-5009 Bergen, Norway
3 Institute of Structural Biology, CEA-CNRS-UJF, 41 Rue Jules Horowitz, 38027 Grenoble, France
Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM
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* Address correspondence to this author at the Department of Biology, Uni-versity of Bergen, P.O. Box 7800, N-5020 Bergen, Norway; Tel: +47 55584882; Fax: +47 55589671; E-mail: Gyri.Haugland@bio.uib.no