The Open Biochemistry Journal

ISSN: 1874-091X ― Volume 13, 2019

Analysis of Endothelial Protein C Receptor Functionality on Living Cells’

E Ducros1, S Mirshahi2, C Bermot1, M Mirshahi*, 1
1 Centre de Recherche des Cordeliers, Université Pierre et Marie Curie – Paris 6, UMRS 872, equipe 18, Paris
2 Stago R&D, Gennevilliers, France


Activated protein C (APC) is a major control system of blood coagulation. APC prevents coagulation pathway by degrading Va and VIIIa plasma’s coagulation factors. Protein C activation requires its binding to specific endothelial cell receptor (EPCR). APC binding to EPCR also activates a wide range of defense mechanisms (anti-inflammatory, antiapoptosis…). EPCR expression by cells can be detected by various methods, including immunoanalysis and molecular biology. However, no assays evaluate its functionality. A method, inspired of a standard fibrinoformation time assay, was developed to estimate EPCR ability to bind APC on living cell surface in vitro. Endothelial cells were incubated with APC and fibrinoformation on cells was followed by spectrophotometry (plasma absorbance increases with fibrin polymerization). Membrane-bound EPCR retain APC, thus prolonging fibrinoformation time in a dose-dependent manner. Control was realized with EPCR-negative cells. This new method can be used on any cell type to study the expression of other coagulation receptors.

Keywords: Thrombosis, activated partial thromboplastin time, endothelial protein C receptor, activated protein C, Endothelial protein C receptor functionality analysis.

Article Information

Identifiers and Pagination:

Year: 2009
Volume: 3
First Page: 49
Last Page: 54
Publisher Id: TOBIOCJ-3-49
DOI: 10.2174/1874091X00903010049

Article History:

Received Date: 11/2/2009
Revision Received Date: 11/3/2009
Acceptance Date: 20/3/2009
Electronic publication date: 13/5/2009
Collection year: 2009

Article Metrics:

CrossRef Citations:

Total Statistics:

Full-Text HTML Views: 1822
Abstract HTML Views: 1070
PDF Downloads: 247
Total Views/Downloads: 3139

Unique Statistics:

Full-Text HTML Views: 575
Abstract HTML Views: 552
PDF Downloads: 158
Total Views/Downloads: 1285
Geographical View

© Ducros et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Centre de Recherche des Cordeliers, Université Pierre et Marie Curie – Paris 6, UMR S 872, Les Cordeliers, Paris, F-75006, France; Tel: 000 33 1 42 34 69 39; E-mail:


"We have always had a fruitful cooperation and long-term experience of publishing with Bentham Open."

"A number of our original papers have appeared in its different journals, as the Bentham Open Publishers provides sufficient research in the field of science and technologies (belonging to the pharmaceutical, biotechnology, biomedical industries etc). The staff of the Bentham Open always treat our papers very seriously and gently during the rutile paper reviewing process, so far we always felt their kindness and highly cooperative support. Today, Bentham Open is providing a great support to science and research, giving opportunities of high standard publishing as well as promoting and enhancing selected papers around the world through communication and exchange of global scientific education."

Kholmirzo T. Kholmurodov
Leading Scientist
FLNP (Frank Laboratory of Neutron Physics) JINR (Joint Institute of Nuclear Research) Moscow region, Russia

Browse Contents

Webmaster Contact:
Copyright © 2019 Bentham Open