The Open Biochemistry Journal

ISSN: 1874-091X ― Volume 14, 2020

Leukocyte Count versus C-Reactive Protein Levels in Obese Portuguese Patients Aged 6-12 Years Old

Henrique Nascimento*, 1, 2, Susana Rocha1, 2, Carla Rego3, Helena Ferreira Mansilha4, Alexandre Quintanilha5, Alice Santos-Silva1, 2, Luís Belo**, 1, 2
1 Department of Biochemistry, Faculty of Pharmacy, University of Porto, 4050-047 Porto, Portugal
2 Institute for Molecular and Cell Biology (IBMC), University of Porto, 4150-180 Porto, Portugal
3 Nutrition Unit / Paediatric Service. UAG-MC. Hospital de S. João E.P.E. Faculty of Medicine of Porto University; 4200-319 Porto, Portugal
4 Paediatric Department of Maria Pia Children’s Hospital; 4050-111, Porto, Portugal
5 Institute of Biomedical Sciences of Abel Salazar (ICBAS), University of Porto, 4099-003 Porto, Portugal



to evaluate whether total and differential WBC counts are altered in young obese patients (aged 6-12 years) and if a relationship exists between WBC counts and the severity of obesity as well as with CRP level.

Materials and Methods:

a group of 77 obese patients [32 males and 45 females] and 19 controls [7 males and 12 females] were studied. Total WBC count was performed by using an automatic blood cell counter. Blood cell morphology and WBC differential count were evaluated in Wright stained blood films. The plasma levels of CRP were evaluated by immunoturbidimetry.


obese participants presented with a statistically significant higher neutrophil percentage and CRP levels when compared to controls; the median CRP value was about 5 times higher than that observed in controls. Absolute neutrophil count and neutrophil/lymphocyte ratio were also higher in patients, though without statistical significance. The parameters that were statistically significant related with adiposity markers were neutrophil count and CRP levels. The neutrophil count was positively and statistically correlated with body mass index (BMI), BMI z-score, waist circumference and waist/height ratio, and also with CRP levels. In multiple regression analysis, the only variable that remained statistically associated with neutrophil count was CRP (neutrophil count = 2.612 + 0.439lnCRP; standardised coefficient/beta: 0.384, P=0.001). When performing multiple regression without CRP, the only variable that remained statistically associated with neutrophil count was BMI.


our results demonstrated in obese patients aged 6-12 years, a significant change in the differential leukocyte count towards neutrophilia, together with a significant higher CRP concentration, and that absolute neutrophil count correlates with obesity markers and with CRP levels. Our data also indicate that neutrophil count, a current clinically used low-cost parameter, may be used as an obesity-related inflammatory marker in young obese patients.

Keywords: Leukocytes, C-reactive protein, children obesity.

Article Information

Identifiers and Pagination:

Year: 2010
Volume: 4
First Page: 72
Last Page: 76
Publisher Id: TOBIOCJ-4-72
DOI: 10.2174/1874091X01004010072

Article History:

Received Date: 10/1/2010
Revision Received Date: 26/2/2010
Acceptance Date: 4/3/2010
Electronic publication date: 10/6/2010
Collection year: 2010

© Nascimento et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: // which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to these authors at the Departamento de Bioquímica, Faculdade de Farmácia, Universidade do Porto, 4050-047 Porto, Portugal; Tel: +351-222078906; Fax: +351-222003977; E-mail:** Departamento de Bioquímica, Faculdade de Farmácia, Universidade do Porto, 4050-047 Porto, Portugal; Tel: +351-222078906; Fax: +351-222003977; E-mail:

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