The Open Biochemistry Journal




ISSN: 1874-091X ― Volume 13, 2019

Histidine 117 in the His-Gly-Ser-Asp motif is Required for the Biochemical Activities of Nucleoside Diphosphate Kinase of Mycobacterium smegmatis



Muthu Arumugam, Parthasarathi Ajitkumar*
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India

Abstract

Nucleoside diphosphate kinase (NDK), which is widely conserved in both prokaryotes and eukaryotes, maintains a balanced pool of nucleotide triphosphates and their deoxy derivatives. NDKs from bacterial and other systems contain the conserved HGSD motif, where the His residue is required for the biochemical activities, namely the NTPase (AT-Pase and GTPase), NTP synthesising, and autophosphorylation activities of the enzyme. Amino acid sequence homology comparison of the NDK of Mycobacterium smegmatis (MsmNDK) with the NDKs of other bacterial genera showed the presence of H117GSD motif. While the recombinant wild type MsmNDK showed the NTPase, NTP synthesising, and autophosphorylation activities, the H117Q mutation abolished the biochemical activities of the recombinant MsmNDK-H117Q mutant protein in vitro. These observations demonstrate that the H117 residue in the HGSD motif is required for the biochemical activities of MsmNDK.

Keywords: Nucleoside diphosphate kinase, M. smegmatis, Autophosphorylation, ATPase, GTPase, NTP synthesising activity.


Article Information


Identifiers and Pagination:

Year: 2012
Volume: 6
First Page: 71
Last Page: 77
Publisher Id: TOBIOCJ-6-71
DOI: 10.2174/1874091X01206010071

Article History:

Received Date: 18/3/2012
Revision Received Date: 16/4/2012
Acceptance Date: 19/4/2012
Electronic publication date: 27/7/2012
Collection year: 2012

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© Arumugam and Ajitkumar; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.


* Address correspondence to this author at the Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India;Tel: 91-80-22932344; Fax: 91-80-23602697;E-mail: ajit@mcbl.iisc.ernet.in


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