RESEARCH ARTICLE


Talin-1 Gene Expression as a Tumor Marker in Hepatocellular Carcinoma Patients: A Pilot Study



Amal A. Mohamed1, Naglaa El-Toukhy2, Doaa M. Ghaith3, Ingy Badawy4, Sara M. Abdo5, Mahmoud Elkadeem6, Mohamed N. Mahrous7, Sherief Abd-Elsalam6, *
1 Department Molecular Biology and Biochemistry, Biochemistry, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt
2 Department of Gastroenterology and Infectious Diseases, Hepatology, Faculty of MedicineBenha University, Benha, Egypt
3 Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
4 Faculty of Biotechnology, Misr University for Science and Technology, Cairo, Egypt
5 Department of Chemistry, Division of Biochemistry, Faculty of Science, Helwan University, Cairo, Egypt
6 Department of Tropical Medicine, Tanta University, Tanta, Egypt
7 Shebin-Elkoum Fever Hospital, Shebeen-elkoum, Egypt

Article Information


Identifiers and Pagination:

Year: 2020
Volume: 10
First Page: 15
Last Page: 22
Publisher ID: TOBIOMJ-10-15
DOI: 10.2174/1875318302010010015

Article History:

Received Date: 20/12/2019
Revision Received Date: 29/02/2020
Acceptance Date: 11/03/2020
Electronic publication date: 23/04/2020
Collection year: 2020

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Creative Commons License
© 2020 Mohamed et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correspondence: Address correspondence to this author at the Department of Tropical Medicine, Faculty of Medicine, Tanta University, El Geish Street, Tanta, Gharbia Governorate Egypt; E-mail: sherif_tropical@yahoo.com


Abstract

Background & Aims:

Hepatocellular Carcinoma (HCC) is the most common primary liver tumor. It is the second most common cancer in men and the sixth in women in Egypt. One of the proteins participating in the trans-endothelial migration is Talin-1. It also has a role in the formation and metastasis of different types of cancer. This study aimed to evaluate the diagnostic impact of Talin-1 gene expression in HCC Egyptian patients.

Methods:

Our study included forty HCC patients, thirty liver cirrhosis patients without HCC and thirty healthy subjects. For all groups, clinical and biochemical parameters were investigated. Tumor characteristics were assessed and tumor staging was done using Okuda, CLIP, VISUM and Tokyo staging systems. In addition, Serum Alpha-Fetoprotein (AFP) levels were assayed using Enzyme Immunoassay (EIA) and Talin-1 gene expression was assessed in the Peripheral Blood Mononuclear Cells (PBMCs) via quantitative real-time Polymerase Chain Reaction (PCR).

Results:

Talin-1 gene expression was significantly upregulated in HCC patients in comparison to cirrhotic and control subjects. The Receiver Operating Characteristic (ROC) analysis indicated that Talin-1 gene expression surpasses serum levels of AFP in the diagnosis of HCC. In particular, the cut off value of 9.5 (2-∆∆Ct) recorded an AUC of 85.7% with a sensitivity of 93.3% and specificity of 80%.

Conclusion:

Our data confirmed an évident diagnostic role of Talin-1 gene expression for HCC detection.

Keywords: Hepatocellular carcinoma, Talin-1, Alpha-fetoprotein, Liver cirrhosis, Gene expression, Tumour, Marker.