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The analysis of genomic DNA is widely-used for research, forensic and diagnostic purposes. Here we describe
reliable methods for isolation of cell-free DNA and cellular DNA from urine. Both DNA fractions are suitable for PCR
and Methylation-Specific PCR (MSP) amplification, leading to consistent and reproducible results. A kinetics analysis describes
the decline of efficiency of MSP performed with urinary DNA which had been stored at room temperature with
and without proteinase K for various time periods.