Spectroscopic Study of the Effects of Bioprotectant Systems on the Protein Stability
S Magazù*, 1, F Migliardo1, 2, AJ Ramirez-Cuesta3, MTF Telling3
1 Dipartimento di Fisica, Università di Messina, S. Sperone C.da Papardo, P.O.Box 55 - 98166 Messina, Italy
2 Laboratoire de Dynamique et Structure des Matériaux Moléculaires, University of Lille 1, UMR CNRS 8024 - 59655 Villeneuve d’Ascq CEDEX, France
3 ISIS Facility, Rutherford Appleton Laboratory, Chilton, Oxon, OX11 0QX, UK
Abstract
In the present article the effect of kosmotrope compounds, i.e. systems having the capability to stabilize biological macromolecules, is investigated by using complementary techniques. The attention is focused on the kosmotrope character of trehalose, a glucose disaccharide, compared to its homologous maltose and sucrose. Complementary techniques of neutron scattering, such as Inelastic Neutron Scattering (INS) and Quasi Elastic Neutron Scattering (QENS) allow to point out the capability of trehalose to strongly affect both the structural and dynamical properties of water. Finally the stabilization effect of trehalose on a well know protein, lysozyme, is studied as a function of temperature by Small Angle Neutron Scattering (SANS).
Article Information
Article History:
Received Date: 09/9/2008
Revision Received Date: 17/8/2009
Acceptance Date: 01/10/2009
Electronic publication date: 15/01/2010
Collection year: 2010
© Magazù et al.; Licensee Bentham Open.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (
http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the Dipartimento di Fisica, Università di Messina, P.O. Box 55, S.ta Sperone C.da Papardo, 98166 Messina, Italy; Tel: +390906765025; Fax: +39090395004;
E-mail:
smagazu@unime.it
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Manuscript submitted on 09-9-2008 |
Original Manuscript |
Spectroscopic Study of the Effects of Bioprotectant Systems on the Protein Stability |