REVIEW ARTICLE


Identification of Strain-Specific Genes in Using a Modified HiCEP Method



Yasuo Mitani1, Tsuneaki Watanabe2, Kazuhiro Kondo2, Tomohiro Tamura1, 3, *
1 Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
2 AISIN COSMOS R & D CO. LTD., Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan and
3 Laboratory of Molecular Environmental Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan


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Creative Commons License
© 2007 Mitani et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan Tel: +81-11-857-8938; Fax: +81-11-857-8980; E-mail: t-tamura@aist.go.jp


Abstract

The genus Rhodococcus exhibits a broad catabolic diversity that is often related to unusual genome diversity. A modified HiCEP method was applied for the identification of strain-specific genes by comparing two different strains. Rhodococcus erythropolis JCM3201 and NI86/21 were compared and 11 genes were identified as NI86/21-specific genes.

Keywords: Rhodococcus, strain-specific, plasmid, comparative genomics, modified HiCEP.