RESEARCH ARTICLE
Quartz Crystal Microbalance Detection of Aflatoxin B1 by Self-Assembled Monolayer Technique
Raya Raykova1, *, Dessislava A. Marinkova1, Vera A. Semerdzhieva1, Magalie Michiel2, Pascal Griesmar3, Milena Mourdjeva3, Spaska Yaneva4, Ivo T. Iliev 4
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
Issue: Suppl-1, M3
First Page: 122
Last Page: 128
Publisher ID: TOBIOTJ-13-122
DOI: 10.2174/187407070190130122
Article History:
Received Date: 05/04/2019Revision Received Date: 15/07/2019
Acceptance Date: 13/10/2019
Electronic publication date: 16/10/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
New biosensor techniques allowing detection of low concentrated substances show a great variety nowadays. The construction of a system with modified quartz as a part of a Quartz Crystal Microbalance (QCM) techniques helps the detection and confirmation of low toxin concentrations in a sample.
Objective:
The study aims to allow the application of methods for preparation and modification of the gold surface of piezoelectric crystal for detection of aflatoxin B1(AFB1) in the concentration range 0.2 - 2.0 µg/L by QCM technique (quartz - crystal microbalance).
Methods:
The procedure for the preparation of quartz crystal sensors for experimental purposes was performed. The quartz surface was activated and covered with self-assembled monolayer to immobilize antibody (rabbit anti-aflatoxin B1) for the detection of antigen - antibody reaction.
Results:
The G” corresponds to viscous properties of the material, during applied deformation of the material in the presence of different concentrations, which revealed in the sensitivity of the used resonator.
Conclusion:
Detection of toxic pollutants may be achieved via QCM methods, ultrasound resonator and piezoelectric quartz techniques for measurement. These techniques allow detection of significantly low concentrations of toxic pollutants, in particular, AFB1, compared to analysis with direct and indirect ELISA immunoassays.