Ultrasonography uses microbubbles for enhanced imaging. We created microbubbles that have
preferential adherence to amyloid protein by utilizing the affinity of serum amyloid component P (SAP) to amyloid along
with avidin-biotin interactions.
Biotin-labeled albumin was incorporated into the albumin shell of fluorocarbon gas-filled bubbles. The bubble
was attached through a bridge with biotin incorporated into the shell of the bubble and incubated with avidin-labeled SAP
which was pre-bound to SA (“synthetic amyloid”). This resulted in a bubble targeted to amyloid. The bubbles were evaluated
using fluorescent microscopy and fluorescence-activated cell sorting (FACS).
Microbubbles with a protein shell were bound to amyloid by utilizing the affinity of SAP for amyloid and biotinavidin
We introduce microbubbles specifically targeted to amyloid deposits and intended as future mediators of ultrasound
detection of amyloid deposits and amyloid-selective drug delivery.