Modeling of Amino Acid Separation Process in Single and Dual Chiral Ligand Exchange Chromatographic Systems
Pepa Dimitrova, Hans-Jörg Bart*TU Kaiserslautern, Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern, Germany
Abstract
In the present paper the separation of three amino acids applying ligand exchange chromatography in single and dual chiral systems was modeled using the dispersive equilibrium model. The investigated systems consist of a commercially available non chiral column and a commercially available chiral column using a chiral mobile phase. With the experimentally determined model parameters and the multi-component competitive Langmuir adsorption isotherm a very good mathematical prediction of the break-through curves and analytical injections at low concentrations was achieved. At higher concentrations the model application is limited due to displacement effects and system complexity.
Keywords: Chiral dual system, ligand exchange chromatography, separation modeling.
Article Information
Article History:
Received Date: 30/5/2011
Revision Received Date: 30/7/2011
Acceptance Date: 11/8/2011
Electronic publication date: 30/8/2011
Collection year: 2011
© Dimitrova and Bart; Licensee Bentham Open.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (
http: //creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the TU Kaiserslautern,
Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern,
Germany; Tel: +49 6312052414; Fax: +49 6312052119;
E-mail:
bart@mv.uni-kl.de
Open Peer Review Details |
Manuscript submitted on 30-5-2011 |
Original Manuscript |
Modeling of Amino Acid Separation Process in Single and Dual Chiral Ligand Exchange Chromatographic Systems |