The Open Dentistry Journal

ISSN: 1874-2106 ― Volume 14, 2020

Antibacterial Effect of Peruvian Propolis Collected During Different Seasons on the Growth of Streptococcus Mutans

Thalia B. Becerra1, Roger D. Calla-Poma2, Margarita F. Requena-Mendizabal2, Pablo A. Millones-Gómez3, *
1 Department of Odontology, Catholic University Los Angeles of Chimbote, Chimbote, Perú
2 Department of Oral Rehabilitation, Faculty of Dentistry, National University of San Marcos, Lima, Peru
3 Vice-Rectorate for Investigation, Universidad Alas Peruanas, Lima, Perú



Propolis is a gummy, resinous substance made by bees from the buds and exudates of plants. The antibacterial activity of propolis has been widely studied and is known to vary according to its geographical origin, the type of surrounding flora, the collecting bee species, the mode of its collection and even the season in which it is collected. Unfortunately, these observations have not been corroborated experimentally.


To compare the antibacterial activities of ethanolic extracts of propolis collected in the summer and autumn on the growth of Streptococcus mutans ATCC 25175.

Materials and Methods:

Propolis samples were collected in the summer and autumn and labeled “A” or “B” by an individual who was not directly involved in the study. Then, 5% ethanolic extracts of propolis were prepared for each sample. S. mutans was plated onto brain heart infusion agar plates into which wells were formed, and the plates were divided into four groups to test the antibacterial effectiveness of both the extracts and the positive (0.12% chlorhexidine digluconate) and negative (96% ethanol) controls.


Inhibition halos of 26.4±2.6 and 18.2±1.8 mm were observed for the autumn and summer propolis extracts, respectively, while those of the negative and positive controls were 0 and 13 mm, respectively. These differences were statistically analyzed using Student’s t-test.


The significantly higher growth of S. mutans in the extracts made from propolis collected in autumn than that grown on extracts collected in summer indicates that the season in which propolis is collected does indeed influence its antibacterial activity.

Keywords: Peruvian propolis, Seasons, S. mutans, Antibacterial effectiveness, β-glucosidase, Polyethylene bags.

Article Information

Identifiers and Pagination:

Year: 2019
Volume: 13
First Page: 327
Last Page: 331
Publisher Id: TODENTJ-13-308
DOI: 10.2174/1874210601913010327

Article History:

Received Date: 23/05/2019
Revision Received Date: 24/07/2019
Acceptance Date: 30/08/2019
Electronic publication date: 30/08/2019
Collection year: 2019

© 2019 Becerra et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Vice-Rectorate for Investigation, Universidad Alas Peruanas, Avenida Paseo de la República 5717, Miraflores, Lima, Perú; Tel: 51944908425; E-mail:

Track Your Manuscript:


"Thank you for your magnificent and marvelous support throughout the publication stages. I would like to endorse my experience with you as a Junior Researcher and a recent graduate of the Dental school. Once again Thank you for your Great Help and Guidance throughout the stages of Publication. You guys are a great team and I am proud to be a Young Bentham author."

Asra Sabir Hussain
The University of Edinburgh Business School, UK

"My experience with Bentham Open was a valuable one because the quality of the services and analysis of my paper contributed in improving what we intended to convey to the readers."

José Ricardo Kina
Department of Surgery and Integrated Clinic,
Araçatuba School of Dentistry, São Paulo State University

"The Publication Manager was very cooperative and replied my mails and guided me without any delays; however the reviewing process was too long."

Mahtab Memarpour
Prevention of Oral and Dental Disease Research Center,
Department of Pediatric Dentistry, School of Dentistry,
Shiraz University of Medical Sciences, Shiraz,

Browse Contents

Webmaster Contact:
Copyright © 2020 Bentham Open