The Open Dentistry Journal

ISSN: 1874-2106 ― Volume 14, 2020

Predictive Role of p53 Protein as a Single Marker or Associated with ki67 Antigen in Oral Leukoplakia: A Retrospective Longitudinal Study

Davide B Gissi, Andrea Gabusi, Dora Servidio, Fabio Cervellati , Lucio Montebugnoli*
Department of Biomedical and Neuromotor Sciences, Section of Oral Science, University of Bologna, Italy


Oral leukoplakia (OL) is the most common potentially malignant lesion of the oral cavity. Immunohistochemical analysis of p53 and Ki67 proteins is a simple and inexpensive method widely used in non-dysplastic OLs to reveal lesions predicted to develop oral cancer. The present longitudinal study evaluated the predictive role of p53 and Ki67 proteins alone or in combination in a group of OLs without dysplasia followed for many years. Seventy-seven OL patients referred to our Department between January 2006 and October 2013 underwent histochemical analysis of p53 and Ki67 expression. OLs were considered at high risk in the presence of either high p53 expression (>20%), or low/normal p53 expression associated with high Ki67 expression (Ki67/p53 ratio >3). Seven OLs evolved to OSCC during the follow-up period. Three cases had p53 overexpression, while four had a high Ki67/p53 ratio. Statistical significance was reached when samples with p53 overexpression were combined with samples with high Ki67/p53 ratio (Chi square 5.3; p<0.02). The combined immunohistochemical expression of p53 and Ki67 proteins could be a useful and simple molecular marker for early detection of non-dysplastic OLs at risk of developing oral cancer.

Keywords: Ki67 protein, oral leukoplakia, oral squamous cell carcinoma, p53 protein. .

Article Information

Identifiers and Pagination:

Year: 2015
Volume: 9
First Page: 41
Last Page: 45
Publisher Id: TODENTJ-9-41
DOI: 10.2174/1874210601509010041

Article History:

Received Date: 12/11/2014
Revision Received Date: 1/12/2014
Acceptance Date: 17/12/2014
Electronic publication date: 30 /1/2015
Collection year: 2015

© Gissi et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Oral Sciences, University of Bologna, Via S. Vitale 59 40125 Bologna, Italy; Tel: 0512088136; Fax: 051225208; E-mail:


"Thank you for your magnificent and marvelous support throughout the publication stages. I would like to endorse my experience with you as a Junior Researcher and a recent graduate of the Dental school. Once again Thank you for your Great Help and Guidance throughout the stages of Publication. You guys are a great team and I am proud to be a Young Bentham author."

Asra Sabir Hussain
The University of Edinburgh Business School, UK

"My experience with Bentham Open was a valuable one because the quality of the services and analysis of my paper contributed in improving what we intended to convey to the readers."

José Ricardo Kina
Department of Surgery and Integrated Clinic,
Araçatuba School of Dentistry, São Paulo State University

"The Publication Manager was very cooperative and replied my mails and guided me without any delays; however the reviewing process was too long."

Mahtab Memarpour
Prevention of Oral and Dental Disease Research Center,
Department of Pediatric Dentistry, School of Dentistry,
Shiraz University of Medical Sciences, Shiraz,

Browse Contents

Webmaster Contact:
Copyright © 2020 Bentham Open